Simultaneous assay of glucose, lactate, L-glutamate and hypoxanthine levels in a rat striatum using enzyme electrodes based on neutral red-doped silica nanoparticles
作者： Fen-Fen Zhang , Qiao Wan , Chen-Xin Li , Xiao-Li Wang , Zi-Qiang Zhu
DOI: 10.1007/S00216-004-2804-X
关键词:
摘要: An electrochemical method suitable for the simultaneous measurement of cerebral glucose, lactate, L-glutamate and hypoxanthine concentrations from in vivo microdialysis sampling has been successfully performed first time using a neutral red-doped silica (NRDS) nanoparticle-derived enzyme sensor system. These uniform NRDS nanoparticles (about 50±3 nm) were prepared by water-in-oil (W/O) microemulsion method, characterized TEM technique. The interior maintained its high electron-activity, while exterior nano-silica surface prevented mediator leaching out into aqueous solution, showed biocompability. then mixing with glucose oxidase (GOD), lactate (LOD), (L-GLOD) or xanthine (XOD), immobilized on four glassy carbon electrodes, respectively. A thin Nafion film was coated layer to prevent interference molecules such as ascorbic acid uric dialysate. sensitivity modified electrode system enables monitoring trace levels L-glutamate, diluted dialysate samples rat striatum.
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