Probing Low Affinity and Multivalent Interactions with Surface Plasmon Resonance: Ligands for Concanavalin A
作者： David A. Mann , Motomu Kanai , Dustin J. Maly , Laura L. Kiessling
DOI: 10.1021/JA9818506
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摘要: The affinities of the carbohydrate-binding protein concanavalin A (Con A) for mono- and multivalent ligands were measured by surface plasmon resonance (SPR) detection. Assessing protein−carbohydrate is typically difficult due to weak observed complications that arise from importance multivalency in these interactions. We describe a convenient method rapidly evaluate inhibitory constants panel different ligands, both monovalent multivalent, low-affinity receptors, such as Con A. nonnatural, mannose-substituted glycolipid was synthesized, self-assembled monolayers varying carbohydrate density generated. synthetic surfaces bind Competition experiments employed solution yielded Ki values similar equilibrium binding obtained titration microcalorimetry experiments. In addition, this assay could be used examine various polymeric defined le...
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