摘要: Immunostimulatory sequence-specific RNA oligonucleotides corresponding to 3' terminal sequences of single-stranded minus-sense genomic RNAs are provided. Also provided compositions and methods relating an immunostimulatory 4-mer motif as 5'-C/U-U-G/U-U-3'. Incorporation this short is sufficient confer new altered properties in existing oligonucleotides, including CpG oligodeoxynucleotides. for use the DNA:RNA chimeric invention induce immune response vitro vivo, well treat allergy, asthma, infection, cancer a subject. Single-stranded oligoribonucleotides believed signal through Toll-like receptor (TLR) chosen from TLR9, TLR8, TLR7, TLR3. The can also be used method screen TLR antagonists.
google.it 参考文章(63)
Gonda I, Aerosols for delivery of therapeutic and diagnostic agents to the respiratory tract. Critical Reviews in Therapeutic Drug Carrier Systems. ,vol. 6, pp. 273- 313 ,(1990)
J. Fernando Bazan, Fernando L. Rock, Gerard T. Hardiman, Robert A. Kastelein, Stephen W.K. Ho, Yong-Jun Liu, Human Receptor Proteins; Related Reagents and Methods ,(2001)
Robert L Bratzler, Arthur M Krieg, Stefan Bauer, Christian Schetter, Marion Jurk, Joerg Vollmer, Methods and products for enhancing immune responses using imidazoquinoline compounds ,(2002)
Michael P. Kriegler, Gene transfer and expression: A laboratory manual ,(1990)
Alfonso R. Gennaro, Remington: The Science and Practice of Pharmacy ,(2003)
Nelson R. Holcomb, Steven M. Lefkowitz, Karen W. Shannon, Patrick J. Collins, Buffer composition and method for hybridization of microarrays on adsorbed polymer siliceous surfaces ,(2001)
Joachim Schorr, Heather L. Davis, Arthur M. Krieg, Method of inducing an antigen-specific immune response by administering a synergistic combination of adjuvants comprising unmethylated CpG-containing nucleic acids and a non-nucleic acid adjuvant ,(2001)
Robert L. Bratzler, Inhibition of angiogenesis by nucleic acids ,(2001)
Chris Meier, Eugen Uhlmann, Methylphosphonic acid ester, process for preparing the same and its use ,(1994)