Oncogenes Activated in Radiation-Induced Rat Skin Tumors

摘要: Male, Sprague-Dawley rats were exposed at four weeks of age to 0.8 MeV electron radiation in single or fractionated doses ranging from 800 1600 rads. A sample 6 tumors was excised 50 74 age. The DNA three carcinomas produced transformed foci by transfection NIH3T3 cells. In 4 transfectants a rat tumor derived K-ras Eco R1 fragment seen Southern blot analysis. cancers showed myc gene amplification each case compared normal liver DNA. Hybridization with an H-ras probe no significant this any the tumors. Northern analysis poly A+ RNA demonstrated major increase expression which active assay. Analogous experiment using same mRNA samples differences between skin and tumor. These data demonstrate simultaneous activation oncogenes ras complementation groups