Quantitative characterization of chitosan in the skin by Fourier-transform infrared spectroscopic imaging and ninhydrin assay: application in transdermal sciences.

摘要: Summary The chitosan has been used as the primary excipient in transdermal particulate dosage form design. Its distribution pattern across epidermis and dermis is not easily accessible through chemical assay limited to radiolabelled molecules via quantitative autoradiography. This study explored Fourier-transform infrared spectroscopy imaging technique with built-in microscope means examine molecular over aid of histology operation. skin was conducted using varying weights, deacetylation degrees, particle sizes zeta potentials, obtained microwave ligation polymer chains at solution state. Both permeation retention characteristics increased use smaller reduced acetyl content size, positive charge density. The ratio epidermal dermal decreased these their accumulation (3.90% 18.22%) raised a greater extent than (0.62% 1.92%). A larger nonetheless did promote passage more chitosan. small increase apparently could fluidize stratum corneum essential dictate into systemic circulation. aided approach introduces new dimension mechanistic aspect delivery.