Dicer is required for female reproductive tract development and fertility in the mouse.

摘要: Dicer encodes a riboendonuclease required for microRNA biosynthesis. was inactivated in Mullerian duct mesenchyme-derived tissues of the reproductive tract mouse, using an Amhr2-Cre allele. Although Amhr2-Cre; conditional mutant males appeared normal and were fertile, females infertile. In adult females, there reduction size oviducts uterine horns. The less coiled compared to controls cysts formed at isthmus near uterotubal junction. Unfertilized, degenerate oocytes commonly found within these cysts, indicating defect embryo transit. Beads transferred into oviduct failed migrate uterus. addition, blastocysts directly uterus did not result pregnancy. Histological analysis demonstrated that contained glandular tissue often few glands remained myometrium, abnormal condition known as adenomyosis. mutants, ectopic expression Wnt4 Wnt5a luminal epithelium (LE) (GE) uterus, Wnt11 ectopically expressed GE. These results demonstrate is necessary postnatal differentiation female tract, suggesting microRNAs are important regulators development fertility.