Serpin-6 expression protects embryonic stem cells from lysis by antigen-specific CTL.
作者： Zeinab Abdullah , Tomo Saric , Hamid Kashkar , Nikola Baschuk , Benjamin Yazdanpanah
DOI: 10.4049/JIMMUNOL.178.6.3390
关键词:
摘要: The immune response to embryonic stem (ES) cells is still poorly understood. In this study, we addressed the adaptive cellular undifferentiated and differentiated ES infected with lymphocytic choriomeningitis virus (LCMV), a vertically transmitted pathogen in mice humans. contrast prevailing view, found that murine express MHC class I molecules, although at low levels. When cocultured LCMV-infected cells, syngeneic but not allogeneic LCMV-specific CTL secrete IFN-γ. Strikingly, do efficiently kill cells. showed high-level expression of serine protease inhibitor 6, an endogenous CTL-derived cytotoxic effector molecule granzyme B. Down-regulation serpin-6 by RNA interference sensitized for CTL-induced cell death. results study suggest present viral Ags are recognized I-restricted manner, yet resist CTL-mediated lysis through 6.
jimmunol.org 参考文章(41)
M.J. Buchmeier, R.M. Welsh, F.J. Dutko, M.B.A. Oldstone, The virology and immunobiology of lymphocytic choriomeningitis virus infection. Advances in Immunology. ,vol. 30, pp. 275- 331 ,(1980) , 10.1016/S0065-2776(08)60197-2
M. B. A. Oldstone, Biology and Pathogenesis of Lymphocytic Choriomeningitis Virus Infection Current Topics in Microbiology and Immunology. ,vol. 263, pp. 83- 117 ,(2002) , 10.1007/978-3-642-56055-2_6
Izhak Kehat, Lior Gepstein, Human embryonic stem cells for myocardial regeneration Heart Failure Reviews. ,vol. 8, pp. 229- 236 ,(2003) , 10.1023/A:1024709332039
John W. McDonald, Xiao-Zhong Liu, Yun Qu, Su Liu, Shannon K. Mickey, Dorothy Turetsky, David I. Gottlieb, Dennis W. Choi, Transplanted embryonic stem cells survive, differentiate and promote recovery in injured rat spinal cord. Nature Medicine. ,vol. 5, pp. 1410- 1412 ,(1999) , 10.1038/70986
O Utermöhlen, A Dangel, A Tárnok, F Lehmann-Grube, Modulation by Gamma Interferon of Antiviral Cell-Mediated Immune Responses In Vivo Journal of Virology. ,vol. 70, pp. 1521- 1526 ,(1996) , 10.1128/JVI.70.3.1521-1526.1996
Bellinda A. Bladergroen, Merel C. M. Strik, Niels Bovenschen, Oskar van Berkum, George L. Scheffer, Chris J. L. M. Meijer, C. Erik Hack, J. Alain Kummer, The Granzyme B Inhibitor, Protease Inhibitor 9, Is Mainly Expressed by Dendritic Cells and at Immune-Privileged Sites Journal of Immunology. ,vol. 166, pp. 3218- 3225 ,(2001) , 10.4049/JIMMUNOL.166.5.3218
Nilabh Shastri, F. Gonzalez, Endogenous generation and presentation of the ovalbumin peptide/Kb complex to T cells. Journal of Immunology. ,vol. 150, pp. 2724- 2736 ,(1993)
Thomas C. Doetschman, Rolf Kemler, Margot Katz, Werner Schmidt, Harald Eistetter, The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium Development. ,vol. 87, pp. 27- 45 ,(1985) , 10.1242/DEV.87.1.27
Michael R. Betts, Jason M. Brenchley, David A. Price, Stephen C. De Rosa, Daniel C. Douek, Mario Roederer, Richard A. Koup, Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation Journal of Immunological Methods. ,vol. 281, pp. 65- 78 ,(2003) , 10.1016/S0022-1759(03)00265-5
Rudolf Deibel, Lymphocytic Choriomeningitis Virus in Man JAMA. ,vol. 232, pp. 501- 504 ,(1975) , 10.1001/JAMA.1975.03250050023010