Surface charge distribution is a determinant of antigen deposition in the renal glomerulus: studies employing 'charge-hybrid' molecules.

摘要: The deposition of antigens and immune complexes (IC) in the renal glomerulus is charge-dependent. demonstration that molecules net anionic charge, but with discrete positively charged regions, exhibit affinity for glomerular basement membrane (GBM) extends this concept. Charge hybrid (polar) were constructed by covalently coupling small polycations (lysozyme or linear poly-L-lysine chains a mean 17 20 residues) to larger polyanions (ovalbumin human serum albumin (HSA]. Although products overall charge they still bound structures. Immunofluorescence studies performed after i.v. injection samples into rats revealed HSA:poly-L-lysine had highest affinity. Radioisotopic measurements showed uptake be function number lysine residues; binding HSA:poly-L-lysine20 was 2.5 times higher than HSA:poly-L-lysine17 (P less 0.01). Prior competing polycation (polyethyleneimine 1200) reduced 75%, indicating charge-based nature interaction. alone effectively eliminated from glomeruli within 72 h. Administration followed anti-HSA antibody induced complex formation capillary wall, giving rise granular immunofluorescence pattern subendothelial subepithelial deposits. Molecules polar structure do occur naturally may contribute glomerulonephritis.