Expression and localization of laminin-5 subunits in the mouse incisor

摘要: Laminin-5 is associated with several epithelial tissues and forms part of the anchoring filaments hemidesmosomes. Recent data have shown that expression laminin-5 subunits impaired in junctional epidermolysis bullosa (JEB), and, these patients, enamel hypoplasia commonly observed. Rodent incisors are continuously growing teeth an asymmetry between their labial lingual sides. Enamel matrix formation restricted to side. We analyzed changes localization (α3, β3, γ2) lower mouse. The apical loop located at end side contained stem cells showed for all subunits. In anterior direction, inner dental (IDE) transiently lost immunoreactivity subunits, whereas transcripts β3 subunit remained IDE. All mRNAs proteins were expressed ameloblasts facing predentine also secretory maturation stage ameloblasts. laminin-5. On side, was continuous from root sheath rests Malassez periodontal ligament. These results suggest spatial temporal regulation correlates histogenesis organ, ameloblast differentiation, plays a role adhesion extracellular (enamel or dentine) areas where basement membrane absent.