Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9
作者： Yuejun Chen , Jingyuan Cao , Man Xiong , Andrew J. Petersen , Yi Dong
DOI: 10.1016/J.STEM.2015.06.001
关键词:
摘要: … gene knockout (iKO) remains challenging. We explored building iKO hPSC lines by combining CRISPR/Cas9-… cassette and remove the drug-resistance gene, thus speeding up the …
core.ac.uk
sci-hub.se 参考文章(52)
J. A. Thomson, Embryonic Stem Cell Lines Derived from Human Blastocysts Science. ,vol. 282, pp. 1145- 1147 ,(1998) , 10.1126/SCIENCE.282.5391.1145
Takahiko Matsuda, Constance L. Cepko, Controlled expression of transgenes introduced by in vivo electroporation Proceedings of the National Academy of Sciences of the United States of America. ,vol. 104, pp. 1027- 1032 ,(2007) , 10.1073/PNAS.0610155104
S. W. Cho, S. Kim, Y. Kim, J. Kweon, H. S. Kim, S. Bae, J.-S. Kim, Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases Genome Research. ,vol. 24, pp. 132- 141 ,(2014) , 10.1101/GR.162339.113
Michelle Christian, Tomas Cermak, Erin L Doyle, Clarice Schmidt, Feng Zhang, Aaron Hummel, Adam J Bogdanove, Daniel F Voytas, None, Targeting DNA Double-Strand Breaks with TAL Effector Nucleases Genetics. ,vol. 186, pp. 757- 761 ,(2010) , 10.1534/GENETICS.110.120717
Rimantas Sapranauskas, Giedrius Gasiunas, Christophe Fremaux, Rodolphe Barrangou, Philippe Horvath, Virginijus Siksnys, The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli Nucleic Acids Research. ,vol. 39, pp. 9275- 9282 ,(2011) , 10.1093/NAR/GKR606
Federico González, Zengrong Zhu, Zhong-Dong Shi, Katherine Lelli, Nipun Verma, Qing V. Li, Danwei Huangfu, An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells Cell Stem Cell. ,vol. 15, pp. 215- 226 ,(2014) , 10.1016/J.STEM.2014.05.018
Jeffrey C Miller, Michael C Holmes, Jianbin Wang, Dmitry Y Guschin, Ya-Li Lee, Igor Rupniewski, Christian M Beausejour, Adam J Waite, Nathaniel S Wang, Kenneth A Kim, Philip D Gregory, Carl O Pabo, Edward J Rebar, An improved zinc-finger nuclease architecture for highly specific genome editing Nature Biotechnology. ,vol. 25, pp. 778- 785 ,(2007) , 10.1038/NBT1319
D. Hockemeyer, R. Jaenisch, Gene Targeting in Human Pluripotent Cells Cold Spring Harbor Symposia on Quantitative Biology. ,vol. 75, pp. 201- 209 ,(2010) , 10.1101/SQB.2010.75.021
Kun Qian, CindyTzu‐Ling Huang, Hong Chen, Lisle W Blackbourn IV, Yuejun Chen, Jingyuan Cao, Lin Yao, Cornall Sauvey, Zhongwei Du, Su‐Chun Zhang, None, A simple and efficient system for regulating gene expression in human pluripotent stem cells and derivatives. Stem Cells. ,vol. 32, pp. 1230- 1238 ,(2014) , 10.1002/STEM.1653
I Matsuo, S Kuratani, C Kimura, N Takeda, S Aizawa, Mouse Otx2 functions in the formation and patterning of rostral head. Genes & Development. ,vol. 9, pp. 2646- 2658 ,(1995) , 10.1101/GAD.9.21.2646