Effect of proline rich 15-deficiency on trophoblast viability and survival.

摘要: Deviations from the normal program of gene expression during early pregnancy can lead to embryonic loss as well dysfunctional placentation, which cause significant morbidity and mortality. Proline rich 15 (PRR15) is a low molecular weight nuclear protein expressed by trophoblast gestation. Lentivirus-mediated knockdown PRR15 mRNA in ovine trophectoderm led demise embryo gestational day 15, providing compelling evidence that critical this precarious window development. Our objective was determine effect on expression, proliferation, survival. The first-trimester human cell line, ACH-3P, infected with control lentivirus or expressing short hairpin (sh)RNA target for degradation, resulting 68% reduction mRNA. Microarray analysis these lines revealed differential genes related cancer, focal adhesion, p53 signaling. These changes included up-regulation GDF15, cytokine increased pregnancies preeclampsia. Viability proliferation decreased PRR15-deficient cells, consistent down-regulation cycle-related CCND1 CDK6 an CCNG2 CDKN1A cells. TNFSF10, tumor necrosis factor superfamily member known induce apoptosis significantly Migration through basement membrane matrix population apoptotic cells present when treated shRNA PRR15. results suggest enhances viability survival implantation placentation.