HMGA2 is regulated by LIN28 and BRCA1 in human placental cells

摘要: The chromatin associated transcription factor HMGA2 is a downstream target of let-7 miRNAs and binds to regulate gene expression. Inhibition by RNA-binding proteins LIN28A LIN28B necessary during early embryogenesis ensure stable expression HMGA2. In addition LIN28, regulated BRCA1/ZNF350/CtIP repressor complex. normal tissues, the complex promoter prevent transcription. However, in many cancers oncomiR miR-182 targets BRCA1, preventing BRCA1 translation allowing for increased Little known about regulation placental development; therefore, we hypothesized that both LIN28 can cells. Using siRNA CRISPR editing techniques, found knockdowns increase levels ACH-3P These cells also demonstrated deficiencies cell differentiation, seemingly differentiating solely towards syncytiotrophoblast sublineage, secreting higher amounts hCG, displaying upregulated ERVW-1. Additionally, knockout caused significant decrease mRNA protein remain same. immunoprecipitation, saw binding We HMGA2's LIN28A/B findings suggest novel role human development.