Cloned mRNA sequences for two types of embryonic myosin heavy chains from chick skeletal muscle. II. Expression during development using S1 nuclease mapping.
作者： P K Umeda , C J Kavinsky , A M Sinha , H J Hsu , S Jakovcic
DOI: 10.1016/S0021-9258(18)32559-6
关键词:
摘要: We have examined the expression of two embryonic myosin HC mRNAs using cDNA clones (110 and 251) which we previously constructed from RNA isolated 14-day-old chick skeletal muscle. Sequence divergence in 3' nontranslated regions enabled us to analyze differential corresponding S1 nuclease mapping procedure. Clone 251 mRNA is expressed primarily fast muscle, where its transcripts appear be predominant species. This minimally posthatching period, but it not detected adult leg breast Messenger for clone 110 also However, stages development, continues at a low level muscle The these during development strongly indicates that they correspond different genes coding HCs. Other were partially homologous or identified by mapping. Using probes clones, minimum four other developmentally forms detected. Two "neonatal" HCs, while code HCs present respectively. results therefore suggest much greater diversity than reported.
sci-hub.st 参考文章(54)
S.E. Zweig, The muscle specificity and structure of two closely related fast-twitch white muscle myosin heavy chain isozymes. Journal of Biological Chemistry. ,vol. 256, pp. 11847- 11853 ,(1981) , 10.1016/S0021-9258(19)68483-8
Tomoh MASAKI, Immunochemical comparison of myosins from chicken cardiac, fast white, slow red, and smooth muscle. Journal of Biochemistry. ,vol. 76, pp. 441- 449 ,(1974) , 10.1093/OXFORDJOURNALS.JBCHEM.A130586
J H Rader, I L Flink, E Morkin, Thyroid hormone stimulates synthesis of a cardiac myosin isozyme. Comparison of the two-two-dimensional electrophoretic patterns of the cyanogen bromide peptides of cardiac myosin heavy chains from euthyroid and thyrotoxic rabbits. Journal of Biological Chemistry. ,vol. 254, pp. 3105- 3110 ,(1979) , 10.1016/S0021-9258(17)30188-6
Paul D. Wagner, Alan G. Weeds, Studies on the role of myosin alkali light chains. Recombination and hybridization of light chains and heavy chains in subfragment-1 preparations. Journal of Molecular Biology. ,vol. 109, pp. 455- 470 ,(1977) , 10.1016/S0022-2836(77)80023-5
R.A. Chizzonite, A.W. Everett, W.A. Clark, S. Jakovcic, M. Rabinowitz, R. Zak, Isolation and characterization of two molecular variants of myosin heavy chain from rabbit ventricle. Change in their content during normal growth and after treatment with thyroid hormone. Journal of Biological Chemistry. ,vol. 257, pp. 2056- 2065 ,(1982) , 10.1016/S0021-9258(19)68146-9
C J Kavinsky, P K Umeda, A M Sinha, M Elzinga, S W Tong, R Zak, S Jakovcic, M Rabinowitz, Cloned mRNA sequences for two types of embryonic myosin heavy chains from chick skeletal muscle. I. DNA and derived amino acid sequence of light meromyosin. Journal of Biological Chemistry. ,vol. 258, pp. 5196- 5205 ,(1983) , 10.1016/S0021-9258(18)32558-4
F.A. Sréter, M. Bálint, J. Gergely, Structural and functional changes of myosin during development: comparison with adult fast, slow and cardiac myosin. Developmental Biology. ,vol. 46, pp. 317- 325 ,(1975) , 10.1016/0012-1606(75)90108-6
Allan M. Maxam, Walter Gilbert, [57] Sequencing end-labeled DNA with base-specific chemical cleavages Nucleic Acids Part I. ,vol. 65, pp. 499- 560 ,(1980) , 10.1016/S0076-6879(80)65059-9
M. Sivaramakrishnan, M. Burke, The free heavy chain of vertebrate skeletal myosin subfragment 1 shows full enzymatic activity. Journal of Biological Chemistry. ,vol. 257, pp. 1102- 1105 ,(1982) , 10.1016/S0021-9258(19)68313-4
J. Robbins, G.A. Freyer, D. Chisholm, T.C. Gilliam, Isolation of multiple genomic sequences coding for chicken myosin heavy chain protein. Journal of Biological Chemistry. ,vol. 257, pp. 549- 556 ,(1982) , 10.1016/S0021-9258(19)68399-7