Characterization of cDNA and genomic sequences corresponding to an embryonic myosin heavy chain.
作者： M Periasamy , E E Strehler , M A Strehler-Page , R M Wydro , B Nadal-Ginard
DOI: 10.1016/S0021-9258(17)36337-8
关键词:
摘要: Abstract We report here the isolation and characterization of cDNA genomic sequences corresponding to a rat embryonic myosin heavy chain (MHC) protein. This gene, which is present as single copy in genome, comprises about 25 kilobase pairs DNA contains approximately 80% intronic sequences. The MHC gene belongs highly conserved multigene family, exhibits high degree nucleotide amino acid sequence conservation with other sarcomeric genes from nematode man. S1 nuclease mapping experiments using probes show that this transiently expressed during skeletal muscle development. Its mRNA detected fetal early development persists up 2 weeks after birth overlapping expression neonatal adult mRNAs. However, not exception extraocular fibers. transient isoform produced by its sequential replacement MHCs raises interesting questions mechanism controlling isozyme transitions physiological significance individual
sci-hub.st 参考文章(50)
Stuart M. Heywood, Doris S. Kennedy, Translational control in embryonic muscle. Progress in Nucleic Acid Research and Molecular Biology. ,vol. 19, pp. 477- 484 ,(1976) , 10.1016/S0079-6603(08)60939-3
P K Umeda, C J Kavinsky, A M Sinha, H J Hsu, S Jakovcic, M Rabinowitz, Cloned mRNA sequences for two types of embryonic myosin heavy chains from chick skeletal muscle. II. Expression during development using S1 nuclease mapping. Journal of Biological Chemistry. ,vol. 258, pp. 5206- 5214 ,(1983) , 10.1016/S0021-9258(18)32559-6
A Weydert, P Daubas, M Caravatti, A Minty, G Bugaisky, A Cohen, B Robert, M Buckingham, Sequential accumulation of mRNAs encoding different myosin heavy chain isoforms during skeletal muscle development in vivo detected with a recombinant plasmid identified as coding for an adult fast myosin heavy chain from mouse skeletal muscle. Journal of Biological Chemistry. ,vol. 258, pp. 13867- 13874 ,(1983) , 10.1016/S0021-9258(17)43998-6
M Periasamy, D F Wieczorek, B Nadal-Ginard, Characterization of a Developmentally Regulated Perinatal Myosin Heavy-chain Gene Expressed in Skeletal Muscle* Journal of Biological Chemistry. ,vol. 259, pp. 13573- 13578 ,(1984) , 10.1016/S0021-9258(18)90731-3
E E Strehler, V Mahdavi, M Periasamy, B Nadal-Ginard, Intron positions are conserved in the 5' end region of myosin heavy-chain genes. Journal of Biological Chemistry. ,vol. 260, pp. 468- 471 ,(1985) , 10.1016/S0021-9258(18)89755-1
M Periasamy, E E Strehler, L I Garfinkel, R M Gubits, N Ruiz-Opazo, B Nadal-Ginard, Fast skeletal muscle myosin light chains 1 and 3 are produced from a single gene by a combined process of differential RNA transcription and splicing. Journal of Biological Chemistry. ,vol. 259, pp. 13595- 13604 ,(1984) , 10.1016/S0021-9258(18)90735-0
C J Kavinsky, P K Umeda, A M Sinha, M Elzinga, S W Tong, R Zak, S Jakovcic, M Rabinowitz, Cloned mRNA sequences for two types of embryonic myosin heavy chains from chick skeletal muscle. I. DNA and derived amino acid sequence of light meromyosin. Journal of Biological Chemistry. ,vol. 258, pp. 5196- 5205 ,(1983) , 10.1016/S0021-9258(18)32558-4
R M Medford, H T Nguyen, B Nadal-Ginard, Transcriptional and cell cycle-mediated regulation of myosin heavy chain gene expression during muscle cell differentiation. Journal of Biological Chemistry. ,vol. 258, pp. 11063- 11073 ,(1983) , 10.1016/S0021-9258(17)44386-9
Allan M. Maxam, Walter Gilbert, [57] Sequencing end-labeled DNA with base-specific chemical cleavages Nucleic Acids Part I. ,vol. 65, pp. 499- 560 ,(1980) , 10.1016/S0076-6879(80)65059-9
H. Czosnek, U. Nudel, M. Shani, P.E. Barker, D.D. Pravtcheva, F.H. Ruddle, D. Yaffe, The genes coding for the muscle contractile proteins, myosin heavy chain, myosin light chain 2, and skeletal muscle actin are located on three different mouse chromosomes. The EMBO Journal. ,vol. 1, pp. 1299- 1305 ,(1982) , 10.1002/J.1460-2075.1982.TB01314.X