Developmentally regulated expression of vascular smooth muscle myosin heavy chain isoforms.
作者： M Kuro-o , R Nagai , H Tsuchimochi , H Katoh , Y Yazaki
DOI: 10.1016/S0021-9258(18)51457-5
关键词:
摘要: Two types of smooth muscle myosin heavy chain (MHC) isoforms, SM1 and SM2, were recently identified to have different carboxyl termini (Nagai, R., Kuro-o, M., Babij, P., Periasamy, M. (1989) J. Biol. Chem. 264, 9734-9737). SM2 are considered be generated from a single gene through alternative RNA splicing. In this study we investigated expression vascular MHC isoforms during development in rabbits at the mRNA, protein, histological levels. adults, all cells reacted with both anti-SM1 anti-SM2 antibodies on immunofluorescence, suggesting coexpression cell. fetal perinatal rabbits, however, only antibody consistently muscles. Reactivity was negative neonatal blood vessels gradually increased 30 days after birth. These developmental changes level coincided mRNA each isoform as determined by S1 nuclease mapping, indicating that is controlled However, sodium dodecyl sulfate-polyacrylamide gel electrophoresis aortas revealed presence large amount SM2. Interestingly, did not cross-react our immunoblotting. We conclude differentially regulated third type may exist embryonic
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