A Human-Mouse Chimeric Model of Obliterative Bronchiolitis after Lung Transplantation

摘要: Obliterative bronchiolitis is a frequent, morbid, and usually refractory complication of lung transplantation. Mechanistic study obliterative would be aided by development relevant model that uses human immune effector cells airway targets. Our objective was to develop murine chimera mimics allograft recipients in airways vivo. Human peripheral blood mononuclear were adoptively transferred immunodeficient mice lacking activity T, B, NK cells, with without concurrent transplantations small dissected from allogeneic cadaveric lungs. Chimerism T occurred the majority recipient animals. The chimeric became highly activated, rapidly infiltrated into grafts, caused bronchiolitis. In contrast, implanted control did not also receive cell transfers remained intact. vitro proliferation assays indicated had enhanced specific proliferative responses donor alloantigens. This confirms critical role among provides novel easily implemented mechanism for detailed, reductionist vivo studies T-cell airways.