In vitro periodontal ligament fibroblast attachment to plasma-cleaned titanium surfaces.

摘要: The objective of this research was to characterize the in vitro cellular behavior fibroblast-like cells derived from rat periodontal ligament on commercially pure titanium surfaces which were sterilized by a variety treatments. Following standard surface preparation protocols, Ti specimens either steam autoclaving, exposure ethylene oxide gas, ultraviolet light, or plasma-cleaning argon for one min five min. Fibroblast-like serum-supplemented media incubated various up two h. In general, levels cell attachment plasma-cleaned significantly higher than those steam-sterilized surfaces, but lower both ultraviolet-treated and tissue culture plastic control. duration plasma cleaning itself did not have significant effect percentage at any time period. SEM evaluations indicated that h, morphology different variously treated specimens. These studies indicate method sterilization following implant can affect initial biological events spreading.