GLAST: gene expression regulation by phorbol esters.
作者： Mónica Espinoza-Rojo , Esther López-Bayghen , Arturo Ortega
DOI: 10.1097/00001756-200008210-00043
关键词:
摘要: The gene expression regulation of the Na + -dependent high affinity glutamate/aspartate transporter GLAST expressed in cultured Bergmann glia cells from chick cerebellum was studied. A 679bp fragment cDNA cloned and sequenced. Specific PCR primers were used to quantify mRNA levels. Treatment with Ca 2+ /diacylglycerol dependent protein kinase C (PKC) activator, phorbol 12-tetradecanoyl-13.-acetate (TPA) produced a decrease levels, without an effect its half life, suggesting transcriptional down regulation. Activation cAMP pathway results transient contrast TPA effect. These findings suggest that is under control distinct signaling pathways.
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