Stem cell specific epigenetic priming and B cell specific transcriptional activation at the mouse Cd19 locus

摘要: Low-level expression of multiple lineage-specific genes is a hallmark hematopoietic stem cells (HSCs). HSCs predominantly express specific for the myeloid or megakaryocytic-erythroid lineages, whereas transcription lymphoid appears to begin after specification. It has been demonstrated number that epigenetic priming occurs before gene and lineage specification; however, little known about how regulated. To address question B cell-restricted established, we studied activation Cd19 during development. We identified cell-specific upstream enhancer showed developmental regulation involves precisely coordinated alterations in factor binding chromatin remodeling at cis-regulatory elements. In multipotent progenitor cells, first remodeled enhancer, this associated with E2A. This followed by EBF PAX5 B-cell differentiation. The promoter transcriptionally activated only binding. Our experiments give important mechanistic insights into widely expressed factors cooperate mediate hematopoiesis.