hsa_circ_0121582 inhibits leukemia growth by dampening Wnt/β-catenin signaling.

摘要: The prognosis of AML patients with chemotherapy is poor, especially those who are insensitive to and resistant drugs. To clarify the underlying pathogenesis provide new therapeutic targets for clinical treatment, we explore role circRNA in leukemia. High-throughput sequencing analysis was performed leukemia healthy donors. RT-qPCR western blot were used determine expression GSK3β. RNA pull-down assay detect miRNAs pulled down by hsa_circ_0121582. immunoprecipitation evaluate binding capacity between TET1 A highly stable found, which derived from reverse splicing GSK3β exon 1 7, hsa_circ_0121582 down-regulated cells. In gain-of-function experiments, up-regulated inhibited proliferation cells vitro vivo. cytoplasm, could act as a sponge miR-224, attenuate inhibiting effect miR-224 on GSK3β, thus up-regulate level addition, bind promoter nucleus, recruit DNA demethylase ensuring transcription upregulated Wnt/β-catenin signaling pathway, reduced aggregation β-catenin This study found that involved inhibition tumor proliferation, restoration be an effective treatment strategy