A RGG motif protein is involved in Toxoplasma gondii stress-mediated response.

摘要: Abstract The molecular mechanisms controlling gene expression are still poorly understood in apicomplexan parasites. Here, we report the characterization of a homolog single strand binding proteins (named TgSsossB) Toxoplasma gondii. We previously showed that TgSsossB interacts with TgAlba involved translation regulation. examined role stress-mediated response, and particularly its rginine– g lycine– lycine (RGG) repeats domain. recombinant protein is able to bind DNA RNA sequence-independent manner, but not double stranded DNA. RGG motif this ability nucleic acid. produced mutant tagged strain lacking using knock-in strategy. observed exhibited fitness defect compared parental Moreover, fewer plaques stress conditions, due slow growth phenotype when extracellular parasites exposed stress. At level, lost interact TgAlba2 an isoform TgAlba1, indicating complex likely non-functional those Thus, our findings define domain as protein–protein interaction platform underline TgAlba–TgSsossB response.