Genomic library screening for viruses from the human dental plaque revealed pathogen-specific lytic phage sequences

摘要: Bacterial pathogenesis presents an astounding arsenal of virulence factors that allow them to conquer many different niches throughout the course infection. Principally fascinating is fact some bacterial species are able induce diseases by expression combinations factors. Nevertheless, studies aiming at screening for presence bacteriophages in humans have been limited. Such procedures would eventually lead identification phage-encoded properties impart increased fitness and/or a particular niche, and hence, potentially be used reverse infections. As human oral cavity represents rich dynamic ecosystem several upper respiratory tract pathogens. However, little known about virus diversity dental plaque which important reservoir. We applied culture-independent approach characterize making library from DNA fraction amplified using multiple displacement method sequenced 80 clones. The resulting sequence showed 44% significant identities GenBank databases TBLASTX analysis. TBLAST homology comparisons 66% was viral; 18% eukarya; 10% bacterial; 6% mobile elements. These sequences were sorted into 6 contigs 45 single 4 identity small region putative prophage Corynebacterium diphtheria genome. findings interestingly highlight uniqueness over half sequences, whilst dominance pathogen-specific imply their role virulence.