Phosphorylation at Thr167 is required for Schizosaccharomyces pombe p34cdc2 function.

摘要: Abstract Eukaryotic cell cycle progression requires the periodic activation and inactivation of a protein-serine/threonine kinase which in fission yeast is encoded by cdc2+ gene. The activity this gene product, p34cdc2, controlled numerous interactions with other proteins its phosphorylation state. In yeast, p34cdc2 phosphorylated on two sites, one has been identified as Tyr15. Dephosphorylation Tyr15 regulates initiation mitosis. To understand more completely regulation activity, we have second site Thr167, residue conserved amongst all homologues. By analysing phenotypes cells expressing various position 167 mutations performing vitro experiments, establish that Thr167 required for at mitosis involved association cyclin B. might also play role exit from