Lineage- and differentiation-dependent alterations in the expression of receptors for glycoconjugates (lectins) in different human hematopoietic cell lines and low grade lymphomas.

摘要: Important biological functions and cellular recognition phenomena are supposedly governed by specific sugar-protein interactions. Human hematopoietic cell lines offer an excellent model for the study of expression endogenous receptors carbohydrate part glycoconjugates with respect to lineage modulation differentiation. Initially, a panel fluorescent (neo)glycoproteins was successfully employed demonstrate cytologically actual presence such on different lines: B lymphoblast line, Daudi; T lymphoblastic leukemia P 12; multipotent leukemic K 562 promyelocytic HL 060. Biochemical analyses were performed using affinity chromatography supports immobilized lactose asialofetuin (simple or complex β-galactosides), melibiose (α-galactoside), fucose, N-acetyl-D-galactosamine, maltose (α-glucoside), mannose-rich yeast glycoprotein, mannan, glycopeptides containing sialic acid residues heparin. Subsequently, sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis used detect lineage-dependent changes in these parameters. Differentiation-dependent specificity galactose, N-acetylgalactosamine, heparin similarly uncovered upon dimethyl sulfoxide-induced differentiation 60 cells. Differences this type characteristic also apparent lymphoma cells from patients various histological subtypes lowgrade lymphomas. This initial description lineage- differentiation-dependent differences human lymphomas suggests that advances knowledge composition sugar (lectins) may aid understanding aspects behavior their related malignancies via participation (lectin)