Optimizing the production of Polyphosphate from Acinetobacter towneri

摘要: Inorganic polyphosphates (PolyP) are linear polymers of few to several hundred orthophosphate residues, linked by energy-rich phosphoanhydride bonds. Four isolates had been screened from soil sample. By MALDI-TOF analysis, they were identified as Bacillius cereus, Acinetobacter towneri, B. megaterium and cereus. The production PolyP in four was studied phosphate uptake medium sulfur deficient at pH 7. These organisms shown significant after 22h incubation. extracted the cells using alkaline lysis method. Among those isolates, towneri found have high (24.57% w/w P) accumulation medium. media optimization for deficiency carried out Response surface methodology (RSM). It proven that increase level presence glucose, under limiting condition, enhanced these condition can be simulated effective removal wastewater sources.