Endothelium-derived relaxing factor from cultured human endothelial cells inhibits aggregation of human platelets.
作者： Ute Alheid , Jürgen C. Frölich , Ulrich Förstermann
DOI: 10.1016/0049-3848(87)90361-6
关键词:
摘要: The present study was designed to investigate whether endothelium-derived relaxing factor (EDRF) produced by cultured human endothelial cells (HEC) inhibits the aggregation of platelets. Microcarrier beads covered with HEC from umbilical veins ( ∼ 2 × 106) or empty (as controls) were co-incubated in an aggregometer washed platelets 5 107). Indomethacin throughout and no prostacyclin production (measured as 6-keto-PGF1α radioimmunoassay) could be detected. presence markedly inhibited thrombin-induced platelet this inhibition further enhanced bradykinin, a stimulator EDRF production. anti-platelet aggregatory effect blocked treating inhibitor gossypol, soluble guanylate cyclase methylene blue, adding scavenger oxyhemoglobin mixture. antiaggregatory material labile, since supernatant indomethacin-treated did not inhibit aggregation. In absence indomethacin, prostacyclin-mediated blue hemoglobin. These data strongly suggest that formed is may constitute important defense mechanism against vasospasm
elsevier.com
sci-hub.se 参考文章(25)
BT Mellion, LJ Ignarro, EH Ohlstein, EG Pontecorvo, AL Hyman, PJ Kadowitz, Evidence for the inhibitory role of guanosine 3', 5'-monophosphate in ADP-induced human platelet aggregation in the presence of nitric oxide and related vasodilators Blood. ,vol. 57, pp. 946- 955 ,(1981) , 10.1182/BLOOD.V57.5.946.946
CN Chesterman, R Owe-Young, J Macpherson, SA Krilis, Substrate for endothelial prostacyclin production in the presence of platelets exposed to collagen is derived from the platelets rather than the endothelium Blood. ,vol. 67, pp. 1744- 1750 ,(1986) , 10.1182/BLOOD.V67.6.1744.1744
S Moncada, J R Vane, Pharmacology and endogenous roles of prostaglandin endoperoxides, thromboxane A2, and prostacyclin. Pharmacological Reviews. ,vol. 30, pp. 293- 331 ,(1978)
R I Levin, B B Weksler, E A Jaffe, The interaction of sodium nitroprusside with human endothelial cells and platelets: nitroprusside and prostacyclin synergistically inhibit platelet function. Circulation. ,vol. 66, pp. 1299- 1307 ,(1982) , 10.1161/01.CIR.66.6.1299
U. Förstermann, G. Hertting, B. Neufang, The importance of endogenous prostaglandins other than prostacyclin, for the modulation of contractility of some rabbit blood vessels British Journal of Pharmacology. ,vol. 81, pp. 623- 630 ,(1984) , 10.1111/J.1476-5381.1984.TB16127.X
Hiroshi Azuma, Masayuki Ishikawa, Satomi Sekizaki, Endothelium‐dependent inhibition of platelet aggregation British Journal of Pharmacology. ,vol. 88, pp. 411- 415 ,(1986) , 10.1111/J.1476-5381.1986.TB10218.X
Ulrich Förstermann, Grit Trogisch, Rudi Busse, Species-dependent differences in the nature of endothelium-derived vascular relaxing factor European Journal of Pharmacology. ,vol. 106, pp. 639- 643 ,(1984) , 10.1016/0014-2999(84)90071-2
D HEARSE, Myocardial protection during ischemia and infarction: Prospects for injury-delaying therapy Journal of Molecular and Cellular Cardiology. ,vol. 18, pp. 18- 18 ,(1986) , 10.1016/S0022-2828(86)80353-4
F Murad, Cyclic guanosine monophosphate as a mediator of vasodilation. Journal of Clinical Investigation. ,vol. 78, pp. 1- 5 ,(1986) , 10.1172/JCI112536
R M Rapoport, F Murad, Agonist-induced endothelium-dependent relaxation in rat thoracic aorta may be mediated through cGMP. Circulation Research. ,vol. 52, pp. 352- 357 ,(1983) , 10.1161/01.RES.52.3.352