Identification of rat prostatic steroid-binding protein as a target antigen of experimental autoimmune prostatitis: implications for prostate cancer therapy.

摘要: The long term goal of this study is to develop autoimmune prostatitis as a therapy for prostate cancer. An immune attack capable destroying normal epithelial cells should also destroy malignant tissue and provide therapeutic benefit in cancer patients. current was initiated identify antigenic targets experimental on the assumption that such proteins might be suitable immunotherapy Male Lewis rats were immunized with syngeneic homogenates, sera used screen immunoreactivity by Western blot analysis. dominant protein recognized purified ion exchange chromatography reverse phase HPLC. Microsequence analysis two polypeptide components immunodominant demonstrated N-terminal sequences identical three component chains rat prostatic steroid-binding (PSBP). T cell responses PSBP detected homogenate. Immunizing male induced vigorous Ab responses. Significant inflammation observed some PSBP. Adoptive transfer rapid severe destructive prostatitis. These results demonstrate major target Ag model may serve vaccine against