Generation of thioarsenicals is dependent on the enterohepatic circulation in rats.

摘要: Three minor sulfur-containing arsenic metabolites: monomethylmonothioarsonic acid (MMMTAV), dimethylmonothioarsinic (DMMTAV), and dimethyldithioarsinic (DMDTAV) were recently found in human animal urine after exposure to inorganic arsenic. However, it remains unclear how the thioarsenicals are formed body then excreted into urine. It is hypothesized that generation of occurs during enterohepatic circulation. To address this hypothesis, male Sprague Dawley (SD) rats Eisai hyperbilirubinuric (EHB) (with deficiency multidrug resistance-associated protein 2) orally administered a single dose arsenite (iAsIII) at 3.0 mg kg−1 weight. Five hours dosing, less than 1.0% was recovered bile EHB rats, while more 27% SD with majority being monomethylarsinodiglutathione [MMA(SG)2] small amount triglutathione [iAs(SG)3]. During early time periods (3 h 6 h) levels liver, red blood cells (RBCs) plasma higher those approximately 76% 87% RBCs respectively, day 5 dosing. there no significant differences concentration between two types animal. Regarding species both rat, thiolated arsenicals MMMTAV DMMTAV detected rat urine, however only low detected. The present result metabolic balance speciation study suggests formation dependent on In addition, vitro experiments indicated from may be transformed by gastrointestinal microbiota DMMTAV, which absorbed bloodstream finally