RpoS and oxidative stress conditions regulate succinyl-CoA: 3-ketoacid-coenzyme A transferase (SCOT) expression in Burkholderia pseudomallei.
作者： Palatip Chutoam , Varodom Charoensawan , Patompon Wongtrakoongate , Apiratana Kum-arth , Pisanu Buphamalai
关键词:
摘要: Burkholderia pseudomallei, a pathogenic gram-negative bacterium, causes the severe human disease melioidosis. This organism can survive in eukaryotic host cells by escaping reactive oxygen species via regulation of stress responsive sigma factors, including RpoS. In B. RpoS has been reported to play role oxidative response through enhanced activity OxyR and catalase. this study, dependent system was further characterized using comparative proteomic analysis. The profiles wild-type pseudomallei following exposure H2O2 between rpoS mutant strains were analyzed. Using stringent criteria, 13 proteins, eight which are regulated RpoS, identified with high confidence. It observed that ScoA, subunit SCOT enzyme not previously shown be involved directly response, is significantly down-regulated after hydrogen peroxide treatment. ScoA ScoB have predicted organized single operon computational methods: study it confirmed RT-PCR these genes indeed co-transcribed as mRNA. present first report for down-regulation expression pseudomallei.
sci-hub.se 参考文章(55)
VI Lushchak, None, Oxidative stress and mechanisms of protection against it in bacteria. Biochemistry. ,vol. 66, pp. 476- 489 ,(2001) , 10.1023/A:1010294415625
Robert Jeanjean, Emmanuel Talla, Amel Latifi, Michel Havaux, Annick Janicki, Cheng-Cai Zhang, A large gene cluster encoding peptide synthetases and polyketide synthases is involved in production of siderophores and oxidative stress response in the cyanobacterium Anabaena sp. strain PCC 7120. Environmental Microbiology. ,vol. 10, pp. 2574- 2585 ,(2008) , 10.1111/J.1462-2920.2008.01680.X
H White, W P Jencks, Properties of succinyl-CoA:3-ketoacid coenzyme A transferase. Journal of Biological Chemistry. ,vol. 251, pp. 1708- 1711 ,(1976) , 10.1016/S0021-9258(17)33706-7
Alberto Boveris, Nozomu Oshino, Britton Chance, The cellular production of hydrogen peroxide Biochemical Journal. ,vol. 128, pp. 617- 630 ,(1972) , 10.1042/BJ1280617
V L Franzon, J Arondel, P J Sansonetti, Contribution of superoxide dismutase and catalase activities to Shigella flexneri pathogenesis. Infection and Immunity. ,vol. 58, pp. 529- 535 ,(1990) , 10.1128/IAI.58.2.529-535.1990
Helena I. M. Boshoff, Clifton E. Barry, Tuberculosis — metabolism and respiration in the absence of growth Nature Reviews Microbiology. ,vol. 3, pp. 70- 80 ,(2005) , 10.1038/NRMICRO1065
Sushil K Jain, Gideon Lim, Pyridoxine and pyridoxamine inhibits superoxide radicals and prevents lipid peroxidation, protein glycosylation, and (Na+ + K+)-ATPase activity reduction in high glucose-treated human erythrocytes. Free Radical Biology and Medicine. ,vol. 30, pp. 232- 237 ,(2001) , 10.1016/S0891-5849(00)00462-7
Patompon Wongtrakoongate, Napachanok Mongkoldhumrongkul, Suthidarak Chaijan, Sumalee Kamchonwongpaisan, Sumalee Tungpradabkul, Comparative proteomic profiles and the potential markers between Burkholderia pseudomallei and Burkholderia thailandensis Molecular and Cellular Probes. ,vol. 21, pp. 81- 91 ,(2007) , 10.1016/J.MCP.2006.08.006
Kevin R. Messner, James A. Imlay, The identification of primary sites of superoxide and hydrogen peroxide formation in the aerobic respiratory chain and sulfite reductase complex of Escherichia coli. Journal of Biological Chemistry. ,vol. 274, pp. 10119- 10128 ,(1999) , 10.1074/JBC.274.15.10119
Vyacheslav S Slyshenkov, Katarzyna Piwocka, Ewa Sikora, Lech Wojtczak, None, Pantothenic acid protects jurkat cells against ultraviolet light-induced apoptosis. Free Radical Biology and Medicine. ,vol. 30, pp. 1303- 1310 ,(2001) , 10.1016/S0891-5849(01)00531-7