Cryopreservation of prostate cancer tissue during routine processing of fresh unfixed prostatectomy specimen: demonstration and validation of a new technique

摘要: BACKGROUND Most molecular techniques currently require fresh frozen tumor tissue, which in the case of prostatectomy specimen is a challenge to obtain for variety intrinsic reasons. Prostate cancers are usually located organ periphery and hence meticulous attention has be paid relation between surgical margin. In this article we describe new technique that allows material rather large quantities without jeopardizing diagnostic accuracy. METHOD An inner triangle, representing roughly 50% entire prostate removed from native cryopreserved, leaving routine histomorphological analysis. We have validated using series 180 archived radical had been studied by histology their entirety, as well 42 worked-up technique. RESULTS The described effective, yielding tissue 84.2% unilateral (≤pT2b) 91.8% bilateral (pT2c) carcinomas. The number containing blocks ranged 1 7 per carcinoma (mean 5.6 ± 3.2). remaining peripheral portion subjected histological analysis allowed estimate total volume with high degree confidentiality (r2 ≥ 0.90 linear regression analysis). routinely examined each significantly reduced, thus saving costs loss accuracy. CONCLUSION The cancer cryoconservtion feasible, highly standardized, safe, economic. 69: 191–197, 2009. © 2008 Wiley–Liss, Inc.