Exome Enrichment and SOLiD Sequencing of Formalin Fixed Paraffin Embedded (FFPE) Prostate Cancer Tissue

摘要: Next generation sequencing (NGS) technologies have revolutionized cancer research allowing the comprehensive study of using high throughput deep methodologies. These methods detect genomic alterations, nucleotide substitutions, insertions, deletions and copy number alterations. SOLiD (Sequencing by Oligonucleotide Ligation Detection, Life Technologies) is a promising technology generating billions 50 bp reads. This robust technique, successfully applied in gene identification, might be helpful detecting novel genes associated with initiation progression formalin fixed paraffin embedded (FFPE) tissue. study’s aim was to compare validity whole exome fresh-frozen vs. FFPE tumor tissue normalization normal prostatic tissue, obtained from same patient. One primary sample, corresponding prostate sample matched adjacent subjected sequencing. The sequenced reads were mapped compared. Our first show comparable results between tissues A prior has been conducted comparing fresh frozen samples other NGS platforms. validation further proves that material reliable source for