Alterations in Cell Surface Carbohydrates on T Cells from Virally Infected Mice Can Distinguish Effector/Memory CD8+ T Cells from Naive Cells

摘要: Glycosylation changes on surface molecules of T cells affect cell trafficking and function may be useful in discriminating between naive, effector, memory cells. To analyze oligosaccharide structures activated vivo, we examined alterations sialic acid residues following infection mice with lymphocytic choriomeningitis (LCMV), vaccinia virus, vesicular stomatitis virus. We found that the majority CD8 from acutely infected these viruses showed increased binding to peanut agglutinin (PNA). All PNAhighCD8 were CD44high, indicating glycosylation occurring There was also an increase PNAhighCD4 population virally mice. Increased PNA correlated higher endogenous neuraminidase levels This activity most likely contributed PNAhigh phenotype by cleaving off core-1 O-glycans or glycoproteins destined for surface. A persisted immune had cleared LCMV infection. When spleen sorted into PNAlow populations, >95% LCMV-specific segregated population. shows virus-specific remain hyposialylated can distinguished naive based binding. Thus, used as a marker Ag-experienced