The distribution of actin in non-muscle cells. The use of actin antibody in the localization of actin within the microfilament bundles of mouse 3T3 cells.

作者: R.D. Goldman , E. Lazarides , R. Pollack , K. Weber

DOI: 10.1016/0014-4827(75)90323-7

关键词:

摘要: Abstract Living mouse 3T3 cells display a complex array of fibrous structures which are visible with phase contrast, Nomarski and polarized light optics. When fixed stained for indirect immunofluorescence actin antibody, the same fibers show intense fluorescence indicating that they contain actin. Electron microscopy reveals these consist submembranous bundles microfilaments located primarily on attached side cells. The results discussed in terms intracellular localization possible contractile system involved motile activities such as cell locomotion.

参考文章(17)
M. Abercrombie, Joan E.M. Heaysman, Susan M. Pegrum, The locomotion of fibroblasts in culture. IV. Electron microscopy of the leading lamella. Experimental Cell Research. ,vol. 67, pp. 359- 367 ,(1971) , 10.1016/0014-4827(71)90420-4
M. R. Adelman, Edwin William Taylor, Further purification and characterization of slime mold myosin and slime mold actin. Biochemistry. ,vol. 8, pp. 4976- 4988 ,(1969) , 10.1021/BI00840A047
R GOLDMAN, E FOLLETT, The structure of the major cell processes of isolated BHK21 fibroblasts Experimental Cell Research. ,vol. 57, pp. 263- 276 ,(1969) , 10.1016/0014-4827(69)90150-5
E.J. Ambrose, The movements of fibrocytes Experimental Cell Research. ,vol. 8, pp. 54- 73 ,(1961) , 10.1016/0014-4827(61)90340-8
V. M. INGRAM, A side view of moving fibroblasts. Nature. ,vol. 222, pp. 641- 644 ,(1969) , 10.1038/222641A0
M. Abercrombie, The bases of the locomotory behaviour of fibroblasts. Experimental Cell Research. ,vol. 8, pp. 188- 198 ,(1961) , 10.1016/0014-4827(61)90348-2
D BARRETT, G ANGELO, Maternal characteristics of hatching enzymes in hybrid sea urchin embryos Experimental Cell Research. ,vol. 57, pp. 159- 166 ,(1969) , 10.1016/0014-4827(69)90137-2
N. K. Wessells, B. S. Spooner, J. F. Ash, M. O. Bradley, M. A. Luduena, E. L. Taylor, J. T. Wrenn, K. M. Yamada, Microfilaments in Cellular and Developmental Processes Science. ,vol. 171, pp. 135- 143 ,(1971) , 10.1126/SCIENCE.171.3967.135
Cheng-Ming Chang, Robert D. Goldman, The localization of actin-like fibers in cultured neuroblastoma cells as revealed by heavy meromyosin binding. Journal of Cell Biology. ,vol. 57, pp. 867- 874 ,(1973) , 10.1083/JCB.57.3.867