In vitro and in vivo evaluation of gelatin-chondroitin sulphate hydrogels for controlled release of antibacterial proteins.
作者： AJ Kuijpers , PB Van Wachem , MJA Van Luyn , LA Brouwer , GHM Engbers
DOI: 10.1016/S0142-9612(00)00064-8
关键词:
摘要: Chemically cross-linked gelatin–chondroitin sulphate (ChS) hydrogels, impregnated in Dacron, were evaluated as drug delivery systems for antibacterial proteins. The gels, plain or with a water-soluble carbodiimide (EDC) and N-hydroxysuccinimide (NHS). release of lysozyme recombinant thrombocidin (rTC-1), an protein derived from human blood platelets, the gelatin–ChS gels Dacron phosphate-buffered saline at 37°C was determined, compared to gelatin gels. incorporation chondroitin into caused marked increase loading capacity, slower rate. relative profiles rTC-1 equal well Furthermore, showed no loss activity after 1 week release. concentration samples surrounding medium function time calculated using mathematical solutions Ficks second law diffusion semi-infinite composite medium, which is schematic representation slab medium. biocompatibility degradation matrices studied implantation subcutaneous pockets rats. mild tissue reaction, almost complete within 18 weeks implantation.
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