Porphyromonas gingivalis FDC381 multiplies and persists within human oral epithelial cells in vitro.

作者: P N Madianos , P N Papapanou , U Nannmark , G Dahlén , J Sandros

DOI: 10.1128/IAI.64.2.660-664.1996

关键词:

摘要: Porphyromonas gingivalis FDC381 replication and persistence within KB epithelial cells in vitro were studied by means of an antibiotic protection assay electron microscopy. Intracellular counts decreased during the first 24 h; showed a threefold increase second day, indicating intracellular multiplication; after 8 days declined to levels approximating 40% initial invasion. The ability P. persist multiply may constitute pathogenic mechanism periodontal disease.

参考文章(28)
Isberg Rr, Pathways for the penetration of enteroinvasive Yersinia into mammalian cells. Molecular biology & medicine. ,vol. 7, pp. 73- 82 ,(1990)
P J Sansonetti, A Ryter, P Clerc, A T Maurelli, J Mounier, Multiplication of Shigella flexneri within HeLa cells: lysis of the phagocytic vacuole and plasmid-mediated contact hemolysis. Infection and Immunity. ,vol. 51, pp. 461- 469 ,(1986) , 10.1128/IAI.51.2.461-469.1986
L Ramakrishnan, S Falkow, Mycobacterium marinum persists in cultured mammalian cells in a temperature-restricted fashion. Infection and Immunity. ,vol. 62, pp. 3222- 3229 ,(1994) , 10.1128/IAI.62.8.3222-3229.1994
J L Gaillard, P Berche, J Mounier, S Richard, P Sansonetti, In vitro model of penetration and intracellular growth of Listeria monocytogenes in the human enterocyte-like cell line Caco-2. Infection and Immunity. ,vol. 55, pp. 2822- 2829 ,(1987) , 10.1128/IAI.55.11.2822-2829.1987
B B Finlay, S Falkow, Common themes in microbial pathogenicity. Microbiological Research. ,vol. 53, pp. 210- 230 ,(1989) , 10.1128/MR.53.2.210-230.1989
P G Detilleux, B L Deyoe, N F Cheville, Penetration and intracellular growth of Brucella abortus in nonphagocytic cells in vitro. Infection and Immunity. ,vol. 58, pp. 2320- 2328 ,(1990) , 10.1128/IAI.58.7.2320-2328.1990
A Amano, T Ishimoto, H Tamagawa, S Shizukuishi, Role of superoxide dismutase in resistance of Porphyromonas gingivalis to killing by polymorphonuclear leukocytes. Infection and Immunity. ,vol. 60, pp. 712- 714 ,(1992) , 10.1128/IAI.60.2.712-714.1992
Hitoshi OSATAKE, Takao INOUÉ, A new drying method of biological specimens for scanning electron microscopy: the t-butyl alcohol freeze-drying method. Archives of Histology and Cytology. ,vol. 51, pp. 53- 59 ,(1988) , 10.1679/AOHC.51.53
E. A. Elsinghorst, L. S. Baron, D. J. Kopecko, Penetration of human intestinal epithelial cells by Salmonella: molecular cloning and expression of Salmonella typhi invasion determinants in Escherichia coli Proceedings of the National Academy of Sciences of the United States of America. ,vol. 86, pp. 5173- 5177 ,(1989) , 10.1073/PNAS.86.13.5173