Penetration and intracellular growth of Brucella abortus in nonphagocytic cells in vitro.

摘要: In pregnant ruminants, Brucella abortus localizes and replicates within the rough endoplasmic reticulum of trophoblastic epithelial cells. this study, Vero cells were exposed to B. investigate its internalization intracellular growth in nonphagocytic A new double-fluorescence staining procedure discriminate between extracellular bacteria was developed. Studies with quantitative bacteriologic culture disrupted host showed that various strains replicated cells, including smooth virulent (strains 2308S 544), attenuated (strain 19), 45/20 2308R) strains. Rough brucellae more adherent entered a greater number Intracellular replication occurred larger percentage (2308S 544) than (19) or (45/20 Differences adhesiveness invasiveness correlated hydrophobicity organism, as measured by hydrocarbon adherence. Ultrastructurally, (2308S) (45/20) consistently found cisternae nuclear envelope. The results suggest transfer is limiting step infection abortus. Images