Association of cancer stem cell markers genetic variants with gallbladder cancer susceptibility, prognosis, and survival

摘要: Genes important to stem cell progression have been involved in the genetics and clinical outcome of cancers. We investigated germ line variants cancer (CSC) genes predict susceptibility efficacy chemoradiotherapy treatment gallbladder (GBC) patients. In this study, we assessed effect SNPs CSC (surface markers CD44, ALCAM, EpCAM, CD133) (molecular NANOG, SOX-2, LIN-28A, ALDH1A1, OCT-4) with GBC prognosis. Total 610 patients 250 controls were genotyped by using PCR-RFLP, ARMS-PCR, TaqMan allelic discrimination assays. Chemotoxicity graded 2-4 200 tumor response was recorded 140 undergoing neoadjuvant chemotherapy (NACT). Differences genotype haplotype frequency distributions calculated binary logistic regression. Gene-gene interaction model analyzed generalized multifactor dimensionality reduction (GMDR). Overall survival Kaplan-Meier curve multivariate Cox-proportional methods. ALCAM Ars1157Crs10511244 (P = 0.0035) significantly associated susceptibility. GMDR analysis, rs1157G>A, EpCAM rs1126497T>C emerged as best significant ALDH1A1 rs13959T>G increased risk grade 3-4 hematological toxicity. SOX-2 rs11915160A>C, OCT-4 rs3130932T>G, NANOG rs11055786T>C found gene-gene for predicting NACT. both recursive partitioning rs1157GA+AA showed higher mortality hazard ratio. gene polymorphisms while OCT-4, an interactive role response.