Monoclonal antibodies to the transformation-specific glycoprotein encoded by the feline retroviral oncogene v-fms.

摘要: Monoclonal antibodies prepared to epitopes encoded by the transforming gene (v-fms) of McDonough strain feline sarcoma virus were used study v-fms-coded antigens in virus-transformed rat and mink cells. These reacted with three different polypeptides (gP180gag-fms, gp140fms, gp120fms), all which shown be glycosylated. Protein blotting [125I]-labeled monoclonal immunoglobulin G's was determine relative steady-state levels these glycoproteins transformed cells showed that gp120 gp140 predominant products. Immunofluorescence assays subcellular fractionation experiments localized molecules cytoplasm quantitative association sedimentable organelles. Thus, differ both chemically topologically from partially characterized products other known oncogenes presumably transform a mechanism.