Bactericidal activity response of blood neutrophils from critically ill patients to in vitro granulocyte colony-stimulating factor stimulation

摘要: Neutrophil function impairment is common in nonneutropenic critically ill patients. Whether granulocyte colony-stimulating factor (G-CSF) may be useful for preventing nosocomial infection these patients debated. The response of blood neutrophils from to G-CSF was investigated vitro. Prospective study, laboratory investigation two intensive care units. 52 without immunosuppression. Neutrophils obtained on the 5th day their unit stay were incubated with and (1, 10, 100 ng/ml). Reactive oxygen species (ROS) release bactericidal activity against Staphylococcus aureus Pseudomonas aeruginosa evaluated. Plasma cytokines (interleukin tumor necrosis α, G-CSF) measured. Median values (25th–75th percentiles) indicated no stimulatory effect neutrophil either organism: S. aureus, 100% (95–109) unstimulated condition 1 ng/ml G-CSF, P. aeruginosa , 102% (98–109) G-CSF. However, wide interindividual variability found, ranging marked inhibition stimulation. Similar found ROS release. No correlations between activities bacterial strain. Inhibition by associated significantly higher plasma interleukin 10 concentrations. levels whose unresponsive suggesting receptor downregulation. vitro varied widely, depending endogenous not predictable based severity scores.