Human mast cells stimulate vascular tube formation. Tryptase is a novel, potent angiogenic factor.
作者： R J Blair , H Meng , M J Marchese , S Ren , L B Schwartz
DOI: 10.1172/JCI119458
关键词:
摘要: The presence of mast cells near capillary sprouting sites suggests an association between and angiogenesis. However, the role in blood vessel development remains to be defined. In attempt elucidate this relationship, we investigated effect human (HMC-1) their products on dermal microvascular endothelial cell (HDMEC) tube formation. Coculture HMC-1 with HDMEC led a dose-response increase network area vascular growth. Moreover, extent neovascularization was enhanced greatly when were degranulated HDMEC. Further examination using antagonists various revealed blunted response (73-88% decrease) formation if specific inhibitors tryptase present. Tryptase (3 microg/ml) directly added caused significant augmentation growth, which suppressed by inhibitors. also induced proliferation dose-dependent fashion (2 pM-2 nM). Our results suggest that act at new secreting tryptase, then functions as potent previously unrecognized angiogenic factor.
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