Cytogenetic and Y chromosome microdeletion screening of a random group of infertile males

摘要: Abstract Objective To assess whether to perform routine cytogenetic and Y chromosome microdeletion screening on all infertile male patients. Design A study of a random group men. Setting University department. Patient(s) In total, 40 patients had azoospermia (21 nonidiopathic), 27 severe oligozoospermia/oligoasthenozoospermia (≤5 × 10 6 /mL) (5 20 (5–20 (6 16 asthenozoospermia nonidiopathic). Many were candidates for intracytoplasmic sperm injection (ICSI). Intervention(s) Collection blood samples from buccal cells one patient. Main outcome measure(s) Karyotype analysis, polymerase chain reaction (PCR) microdeletions, fluorescence in situ hybridization abnormal chromosomes. Result(s) Ten (9.7%) subjects, including nonidiopathic patient, found have an karyotype. Two idiopathic azoospermic missing large portions euchromatin, confirmed by PCR analysis additional patient microdeletion. Conclusion(s) Routine is required but it may be advisable limit with factor infertility /mL).