A New Method for Melanin at Acid pH

作者: Winsome Garvey , Arleen Fathi , Francine Bigelow , Carmencita Jimenez , Blair Carpenter

DOI: 10.1179/HIS.1990.13.4.279

关键词:

摘要: Abstract A simple, reliable, consistent and economical nonbuffered acid method for melanin is presented. After a short presensitization in silver, the sections are treated gum mastic then immersed physical developer consisting of hydroquinone, mastic, very low concentration silver nitrate. The placed thiosulfate counterstained with nuclear fast red. entire procedure takes under 40 minutes to perform. (The J Histotechnology 13:279, 1990)

参考文章(17)
Steve K. Landas, Robert L. Schelper, Fermin O. Tio, J. Wesley Turner, Kenneth C. Moore, Janet Bennett-Gray, Black thyroid syndrome: exaggeration of a normal process? American Journal of Clinical Pathology. ,vol. 85, pp. 411- 418 ,(1986) , 10.1093/AJCP/85.4.411
Raphael L. Warkel, Lee G. Luna, Elson B. Helwig, A Modified Warthin-Starry Procedure at LowpH for Melanin American Journal of Clinical Pathology. ,vol. 73, pp. 812- 815 ,(1980) , 10.1093/AJCP/73.6.812
P. R. Lewis, D. P. Knight, Staining Methods for Sectioned Material ,(1977)
John D. Bancroft, Marilyn Gamble, Theory and Practice of Histological Techniques ,(1990)
Giuseppe Prota, Recent Advances in the Chemistry of Melanogenesis in Mammals Journal of Investigative Dermatology. ,vol. 75, pp. 122- 127 ,(1980) , 10.1111/1523-1747.EP12521344
Giuliano Francioni, Gianfranco Borgioli, Rapid bleach for melanin. Biotechnic & Histochemistry. ,vol. 61, pp. 239- 242 ,(1986) , 10.3109/10520298609109944
P. Szerdahelyi, P. K�sa, A highly sensitive method for the histochemical demonstration of copper in normal rat tissues. Histochemistry and Cell Biology. ,vol. 85, pp. 349- 352 ,(1986) , 10.1007/BF00493488
Alfredo J. A. Barbosa, Lucia P. F. Castro, Ana Margarida, M. F. Nogueira, A Simple and Economical Modification of the Masson-Fontana Method for Staining Melanin Granules and Enterochromaffin Cells Biotechnic & Histochemistry. ,vol. 59, pp. 193- 196 ,(1984) , 10.3109/10520298409113855