Alkaline Peroxide Pretreatment of Corn Stover for Enzymatic Saccharification and Ethanol Production

摘要: Abstract Alkaline hydrogen peroxide (AHP) pretreatment and enzymatic saccharification were evaluated for conversion of corn stover cellulose hemicellulose to fermentable sugars. Corn used in this study contained 37.0±0.2% cellulose, 26.8±0.2% hemicellulose, 18.0±0.1% lignin on dry basis. Under the optimum conditions AHP [2% H2O2 (v/v), pH 11.5, 35°C, 24 h] (10%, w/v) (45°C, 5.0, 72 h) with 0.1% (w/v) Tween 20, a total 558±12 mg sugars was obtained per g stover, which is equivalent 79.5% theoretical sugar yield. Most (82%) removed from stover. The pretreated enzymatically saccharified hydrolyzate fermented by recombinant Escherichia coli strain FBR 5 at 6.5 37°C 96 h produce 23.9±0.9 g ethanol 56.6±1.6 g L an yield 0.42 g/g available 0.24 g/g 67% theoretical...