Ethanol production from alkaline peroxide pretreated enzymatically saccharified wheat straw.

作者: B.C. Saha , M.A. Cotta

DOI: 10.1021/BP050310R

关键词:

摘要: Wheat straw used in this study contained 44.24 +/- 0.28% cellulose and 25.23 0.11% hemicellulose. Alkaline H(2)O(2) pretreatment enzymatic saccharification were evaluated for conversion of wheat hemicellulose to fermentable sugars. The maximum yield monomeric sugars from (8.6%, w/v) by alkaline peroxide (2.15% H(2)O(2), v/v; pH 11.5; 35 degrees C; 24 h) (45 C, 5.0, 120 three commercial enzyme preparations (cellulase, beta-glucosidase, xylanase) using 0.16 mL each preparation per g was 672 4 mg/g (96.7% yield). During the pretreatment, no measurable quantities furfural hydroxymethyl produced. concentration ethanol (per L) pretreated saccharified (66.0 g) hydrolyzate recombinant Escherichia coli strain FBR5 at 6.5 37 C 48 h 18.9 0.9 with a 0.46 available (0.29 g/g straw). 15.1 0.1 0.23 case simultaneous fermentation E. 6.0 h.

参考文章(24)
R. Moletta, J. M. Navarro, J. P. Delgenes, Acid hydrolysis of wheat straw and process considerations for ethanol fermentation by Pichia stipitis Y7124 Process Biochemistry. ,vol. 25, pp. 132- 135 ,(1990)
Bruce S. Dien, Nancy N. Nichols, Patricia J. O'Bryan, Rodney J. Bothast, Development of new ethanologenic Escherichia coli strains for fermentation of lignocellulosic biomass. Applied Biochemistry and Biotechnology. ,vol. 84, pp. 181- 196 ,(2000) , 10.1385/ABAB:84-86:1-9:181
Badal C. Saha, Bruce S. Dien, Rodney J. Bothast, Fuel Ethanol Production from Corn Fiber Current Status and Technical Prospects Applied Biochemistry and Biotechnology. ,vol. 70, pp. 115- 125 ,(1998) , 10.1007/BF02920129
Anders Thygesen, Anne Belinda Thomsen, Anette S. Schmidt, Henning Jørgensen, Birgitte K. Ahring, Lisbeth Olsson, Production of cellulose and hemicellulose-degrading enzymes by filamentous fungi cultivated on wet-oxidised wheat straw Enzyme and Microbial Technology. ,vol. 32, pp. 606- 615 ,(2003) , 10.1016/S0141-0229(03)00018-8
RodneyJ. Bothast, BadalC. Saha, A.Vance Flosenzier, LonnieO. Ingram, Fermentation of L-arabinose, D-xylose and D-glucose by ethanologenic recombinant Klebsiella oxytoca strain P2 Biotechnology Letters. ,vol. 16, pp. 401- 406 ,(1994) , 10.1007/BF00245060
G. L. Miller, Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar Analytical Chemistry. ,vol. 31, pp. 426- 428 ,(1959) , 10.1021/AC60147A030
Badal C. Saha, Rodney J. Bothast, Pretreatment and enzymatic saccharification of corn fiber. Applied Biochemistry and Biotechnology. ,vol. 76, pp. 65- 77 ,(1999) , 10.1385/ABAB:76:2:65
J.M. Fang, R.C. Sun, D. Salisbury, P. Fowler, J. Tomkinson, Comparative study of hemicelluloses from wheat straw by alkali and hydrogen peroxide extractions Polymer Degradation and Stability. ,vol. 66, pp. 423- 432 ,(1999) , 10.1016/S0141-3910(99)00095-6
George X. Pan, Jennifer L. Bolton, Gordon J. Leary, Determination of Ferulic and p-Coumaric Acids in Wheat Straw and the Amounts Released by Mild Acid and Alkaline Peroxide Treatment Journal of Agricultural and Food Chemistry. ,vol. 46, pp. 5283- 5288 ,(1998) , 10.1021/JF980608F