An immunoassay for the detection of triclosan-O-glucuronide, a primary human urinary metabolite of triclosan
作者： Anupama Ranganathan , Shirley J. Gee , Bruce D. Hammock
DOI: 10.1007/S00216-015-8918-5
关键词:
摘要: Triclosan-O-glucuronide (TCSG) is one of the primary urinary metabolites antibacterial compound triclosan or TCS that found in many personal care products and consumer goods. We have developed a competitive, indirect heterologous ELISA for detection target TCSG urine. Such an could be as useful tool to measure this important biomarker human exposure TCS. Immunogens were prepared by conjugating thyroglobulin, via heterobifunctional cross-linkers AEDP 3-[(2-aminoethyl)dithio] propionic acid•hydrochloride TFCS N-[e-trifluoroacetylcaproyloxy]succinimide ester. The coating antigen was direct conjugation bovine serum albumin. Antibodies raised rabbits 2619, 2621 (immunogen TCSG-AEDP-Thy), 2623 TCSG-TFCS-Thy), screened characterized determine their optimal concentrations. optimized ELISA, with antibody 2621, gave IC50 value 2.85 ng/mL, linear range (IC20-IC80) determined 2.6-24.8 ng/mL. Selectivity assay assessed measuring cross-reactivity related congeners such aglycone TCS, triclosan-O-sulfate, triclocarban, polybrominated diphenyl ether derivative, 3-phenoxybenzyl alcohol glucuronide. There virtually no recognition any these cross-reactants. Graphical Abstract Urinary analysis
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