Enhanced hydrogen peroxide generation accompanies the beneficial bioenergetic effects of methylene blue in isolated brain mitochondria.

摘要: Abstract The redox dye methylene blue (MB) is proven to have beneficial effects in various models of neurodegenerative diseases. Here we investigated the MB (100 nM, 300 nM, and 1 μM) on key bioenergetic parameters H2O2 production/elimination isolated guinea pig brain mitochondria under normal as well respiration-impaired conditions. As measured by high-resolution Oxygraph rate resting oxygen consumption was increased, but ADP-stimulated respiration unaffected with any substrates (glutamate malate, succinate, or α-glycerophosphate) used for supporting mitochondrial respiration. In treated inhibitors complex I III moderately significantly increased ATP production, restored ΔΨm, Ca2+ uptake. are consistent transferring electrons from upstream components electron transport chain cytochrome c, which energetically favorable when flow respiratory compromised. On other hand, production Amplex UltraRed fluorimetry all conditions, resting, ATP-synthesizing, mitochondria, each substrate combination Furthermore, it also decreased elimination H2O2. Generation without superoxide formation, observed presence MB, interpreted a result reduction molecular reduced MB. elevated generation impaired should be considered overall oxidative state