Immunochemical quantitation of 3-(cystein-S-yl)acetaminophen adducts in serum and liver proteins of acetaminophen-treated mice

摘要: Using a recently developed enzyme-linked immunosorbent assay specific for 3-(cystein-S-yl)acetaminophen adducts we have quantitated the formation of these in liver and serum protein B6C3F1 male mice dosed with acetaminophen. Administration acetaminophen at doses 50, 100, 200, 300, 400 500 mg/kg to resulted evidence hepatotoxicity (increase levels alanine aminotransferase aspartate aminotransferase) 4 hr treatment groups only. The was not observed receiving 100 200 doses, but above 300 Greater adduct were higher doses. 3-(Cystein-S-yl)acetaminophen also hepatotoxic After dose acetaminophen, reached peak 2 after dosing. By 12 decreased approximately 10% level. In contrast, delayed, reaching sustained 6 dose-response correlation between appearance aminotransferases temporal decrease are consistent hepatic origin detected protein.(ABSTRACT TRUNCATED AT 250 WORDS)