An assessment of the immunological status of patients with renal cell carcinoma based on the relative abundance of T-helper 1- and -2 cytokine-producing CD4+ cells in peripheral blood

摘要: Objective To assess the immunological status of patients with renal cell carcinoma (RCC), by analysing proportion cluster-of-differentiation 4-positive (CD4+) cells showing intracellular cytokine production, i.e. interferon-γ derived from T-helper (Th) 1 and interleukin-4 Th2 cells, among peripheral blood lymphocytes these patients Patients, subjects methods Peripheral samples (5 mL) were collected 36 (mean age 61 years, range 44–78) RCC before after they underwent nephrectomy. The cytokine-producing CD4+ was determined flow cytometric analysis stimulating phorbol 12-myristate 13-acetate, ionomycin brefeldin A, staining fluorescein isothiocyanate-labelled anti-interferon-γ, anti-interleukin-4 anti-immunoglubulin-2b antibodies. results expressed as percentage in population. As a control, obtained 35 healthy volunteers 34 years, 22–49) also analysed. Results producing significantly higher (P < 0.04 P < 0.001, respectively) than controls. Th1/Th2 ratio (i.e. each cytokine) lower (P < 0.001). There significant correlation controls between production (r = 0.489, P < 0.01) but not RCC. high-stage those low-stage (P < 0.05). less nephrectomy (P < 0.01) greater (P < 0.05) nephrectomy; there no such trend RCC. Conclusion An evaluation is useful for assessing RCC; change predominant response Th1 to increasing stage