Biodistribution and Toxicity Studies of PRINT Hydrogel Nanoparticles in Mosquito Larvae and Cells

摘要: Mosquito-borne diseases continue to remain major threats human and animal health impediments socioeconomic development. Increasing mosquito resistance chemical insecticides is a great public concern, new strategies/technologies are necessary develop the next-generation of vector control tools. We propose novel method for that employs nanoparticles (NPs) as platform delivery mosquitocidal dsRNA molecules silence genes cause lethality. Identifying optimal NP chemistry morphology imperative efficient mosquitocide delivery. Toward this end, fluorescently labeled polyethylene glycol NPs specific sizes, shapes (80 nm x 320 nm, 80 5000 200 1000 nm) charges (negative positive) were fabricated by Particle Replication in Non-Wetting Templates (PRINT) technology. Biodistribution, persistence, toxicity PRINT evaluated vitro cell culture vivo Anopheles gambiae larvae following parenteral oral challenge. Following challenge, biodistribution positively negatively charged each size shape was similar; intense fluorescence observed thoracic abdominal regions larval body. Positively more associated with gastric caeca gastrointestinal tract. Negatively persisted through metamorphosis head, body ovaries adults. detected mid- hindgut. efficiently internalized than NPs. trafficked cytosol, but co-localized lysosomes. none tested induced any cytotoxic effects.