MAPK dependence of DNA damage repair: ionizing radiation and the induction of expression of the DNA repair genes XRCC1 and ERCC1 in DU145 human prostate carcinoma cells in a MEK1/2 dependent fashion.
作者： A. Yacoub , Jong Sung Park , Liang Qiao , P. Dent , M. P. Hagan
DOI: 10.1080/09553000110069317
关键词:
摘要: Purpose : To examine the role of mitogen-activated protein kinase (MAPK) signalling on induction by ionizing radiation nucleotide excision repair gene (ERCC1), X-ray cross-complementing group 1 (XRCC1) and radiation-induced DNA damage. Materials methods The expression ERCC1 XRCC1 was examined in DU145 human prostate cancer cells following exposure to radiation. We characterized MAPK dependence this through RT-PCR analysis, Western transcription inhibition measurement activation each promoter. Pre-exposure with specific MEK1/2 inhibitor PD980059 (10 w M) used blunt without suppressing basal levels activity. In addition, we damage measuring micronucleus formation removal nicking activity associated AP sites. Results Irradiation caused a time-dependent, MAPK-dependent increase i...
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